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pcmv ancbe4max spg  (Addgene inc)


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    Structured Review

    Addgene inc pcmv ancbe4max spg
    Pcmv Ancbe4max Spg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+ancbe4max+spg/pCMV_AncBE4max+(Plasmid+%23112094)/bio_rxiv__2024__08__28__610115-171-20-31
    Average 93 stars, based on 70 article reviews
    pcmv ancbe4max spg - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
    Article Snippet: .. In base editing experiments the following plasmids were cloned by restriction enzyme strategies: pCMV-SpG-ABEmax, pCMV-SpG-ABE8e, pCMV-SpG-ABE8.20m, pCMV-NG-ABEmax , pCMV-NG-ABE8e , pCMV-AncBE4max-SpG, pCAG-CBE4max-NG-P2A-EGFP, pCMV-AncBE4max-NG. pCAG-CBE4max-SpRY-P2A-EGFP (#139999) and pCAG-CBE4max-SpG-P2A-EGFP (#139998) were purchased from Addgene. pIVT-CMV-SpG-ABEmaxP2A-EGFP and pIVT-CBE4max-SpRY-P2A-EGFP, used for in vitro RNA transcription, were constructed starting from pCMV-SpG-ABEmax and pCAG-CBE4max-SpRY-P2A-EGFP introducing the ABE/CBE-Cas9 sequence in the pIVT-SpRY-ABE9 plasmid by EcoRI restriction enzyme digestion. ..

    In Vitro:

    Article Title: Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
    Article Snippet: .. In base editing experiments the following plasmids were cloned by restriction enzyme strategies: pCMV-SpG-ABEmax, pCMV-SpG-ABE8e, pCMV-SpG-ABE8.20m, pCMV-NG-ABEmax , pCMV-NG-ABE8e , pCMV-AncBE4max-SpG, pCAG-CBE4max-NG-P2A-EGFP, pCMV-AncBE4max-NG. pCAG-CBE4max-SpRY-P2A-EGFP (#139999) and pCAG-CBE4max-SpG-P2A-EGFP (#139998) were purchased from Addgene. pIVT-CMV-SpG-ABEmaxP2A-EGFP and pIVT-CBE4max-SpRY-P2A-EGFP, used for in vitro RNA transcription, were constructed starting from pCMV-SpG-ABEmax and pCAG-CBE4max-SpRY-P2A-EGFP introducing the ABE/CBE-Cas9 sequence in the pIVT-SpRY-ABE9 plasmid by EcoRI restriction enzyme digestion. ..

    Construct:

    Article Title: Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
    Article Snippet: .. In base editing experiments the following plasmids were cloned by restriction enzyme strategies: pCMV-SpG-ABEmax, pCMV-SpG-ABE8e, pCMV-SpG-ABE8.20m, pCMV-NG-ABEmax , pCMV-NG-ABE8e , pCMV-AncBE4max-SpG, pCAG-CBE4max-NG-P2A-EGFP, pCMV-AncBE4max-NG. pCAG-CBE4max-SpRY-P2A-EGFP (#139999) and pCAG-CBE4max-SpG-P2A-EGFP (#139998) were purchased from Addgene. pIVT-CMV-SpG-ABEmaxP2A-EGFP and pIVT-CBE4max-SpRY-P2A-EGFP, used for in vitro RNA transcription, were constructed starting from pCMV-SpG-ABEmax and pCAG-CBE4max-SpRY-P2A-EGFP introducing the ABE/CBE-Cas9 sequence in the pIVT-SpRY-ABE9 plasmid by EcoRI restriction enzyme digestion. ..

    Sequencing:

    Article Title: Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
    Article Snippet: .. In base editing experiments the following plasmids were cloned by restriction enzyme strategies: pCMV-SpG-ABEmax, pCMV-SpG-ABE8e, pCMV-SpG-ABE8.20m, pCMV-NG-ABEmax , pCMV-NG-ABE8e , pCMV-AncBE4max-SpG, pCAG-CBE4max-NG-P2A-EGFP, pCMV-AncBE4max-NG. pCAG-CBE4max-SpRY-P2A-EGFP (#139999) and pCAG-CBE4max-SpG-P2A-EGFP (#139998) were purchased from Addgene. pIVT-CMV-SpG-ABEmaxP2A-EGFP and pIVT-CBE4max-SpRY-P2A-EGFP, used for in vitro RNA transcription, were constructed starting from pCMV-SpG-ABEmax and pCAG-CBE4max-SpRY-P2A-EGFP introducing the ABE/CBE-Cas9 sequence in the pIVT-SpRY-ABE9 plasmid by EcoRI restriction enzyme digestion. ..

    Plasmid Preparation:

    Article Title: Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
    Article Snippet: .. In base editing experiments the following plasmids were cloned by restriction enzyme strategies: pCMV-SpG-ABEmax, pCMV-SpG-ABE8e, pCMV-SpG-ABE8.20m, pCMV-NG-ABEmax , pCMV-NG-ABE8e , pCMV-AncBE4max-SpG, pCAG-CBE4max-NG-P2A-EGFP, pCMV-AncBE4max-NG. pCAG-CBE4max-SpRY-P2A-EGFP (#139999) and pCAG-CBE4max-SpG-P2A-EGFP (#139998) were purchased from Addgene. pIVT-CMV-SpG-ABEmaxP2A-EGFP and pIVT-CBE4max-SpRY-P2A-EGFP, used for in vitro RNA transcription, were constructed starting from pCMV-SpG-ABEmax and pCAG-CBE4max-SpRY-P2A-EGFP introducing the ABE/CBE-Cas9 sequence in the pIVT-SpRY-ABE9 plasmid by EcoRI restriction enzyme digestion. ..



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